BAIRD-PARKER AGAR BASE (RPF)



a new improved base medium for use with RPF Supplement (SR0122). This conforms to ISO 6888 Part 2¹ for the enumeration of coagulase-positive staphylococci.

Product Overview

Certifications/Compliance Industrial Reference: ISO, NMKL.

Description Baird-Parker Agar Base, RPF

Format Powder

Product Type Agar

Type Media

Quantity 500 g

Yield For 7.1L medium

Unit Size Each

Technique

Surface Inoculation Method

1.    Prepare the RPF Agar plates as directed.

2.    Process the food sample in a stomacher or Waring blender using the recommended sample size and diluent.

3.    Separate plates are inoculated with 0.1ml of the prepared samples and the subsequent decimal dilutions of them.

4.    Incubate at 35°C or 37°C and examine after 24 and 48 hours incubation.

5.    Count all the colonies that have an opaque halo of precipitation around them. Do not limit the count to black colonies.

6.    Report as number of coagulase positive staphylococcus isolated per gram of food.

Pour Plate Method

1.    Prepare the RPF Agar as directed and hold at 48°C.

2.    Process the food sample in a stomacher or Waring blender using the recommended sample size and diluent.

3.    Add 1ml of the prepared sample (initial suspension and subsequent decimal dilution) into each sterile Petri dish.

4.    Add aseptically 20ml of sterile RPF Agar and prepare pour plates.

5.    Incubate at 35°C or 37°C and examine after 24 to 48 hours.

6.    Count all the colonies that have an opaque halo of precipitation around them.

7.    Report as number of coagulase positive staphylococcus isolated per gram of food.

Storage Conditions and Shelf Life

Store the dehydrated medium at 10-30°C and RPF Supplement below 0°C. Use before the expiry date on the label.

Prepared plates of medium are best used freshly prepared^1,2.

Appearance

Dehydrated medium: Straw coloured, free-flowing powder

Prepared medium: Straw coloured gel

Quality Control

Precautions

Colonies of some contaminating organisms growing in close proximity to the coagulase positive colonies may partially digest the coagulase halo reaction.

รายละเอียดสินค้า

รหัสสินค้า : CM0961B

ยี่ห้อ : Oxoid

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