RAPPAPORT-VASSILIADIS (RV) ENRICHMENT BROTH

A selective enrichment broth for the isolation of salmonellae.

Directions

Add 30g (the equivalent weight of dehydrated medium per litre) to 1 litre of distilled water. Heat gently until dissolved completely. Dispense 10ml volumes into screw-capped bottles or tubes and sterilise by autoclaving at 115°C for 15 minutes.

Description

Rappaport-Vassiliadis (RV) Enrichment Broth is based on the formulation described by van Schothorst and Renaud¹ and is recommended as the selective enrichment medium when isolating Salmonella species from food and environmental specimens. It can also be used to isolate Salmonella from human faeces without pre-enrichment but the inoculum must be small. The original formulation described by Rappaport et al² was specifically developed to exploit the four characteristics of Salmonella species when compared with other Enterobacteriaceae.

1. The ability to survive at relatively high osmotic pressures.

2. To multiply at relatively low pH values.

3. To be relatively more resistant to malachite green.

4. To have relatively less demanding nutritional requirements.

Storage conditions and Shelf life

Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Store the prepared medium at 2-8°C.

Appearance

Dehydrated medium: Pale green coloured, free-flowing powder

Prepared medium: Blue coloured solution

Quality control

รายละเอียดสินค้า

รหัสสินค้า : CM0669

ยี่ห้อ : Oxoid

Documents & Downloads

Technical Data Sheet คลิกที่นี่

MSDS คลิกที่นี่

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