BRILLIANCE E. COLI / COLIFORM SELECTIVE AGAR

Brilliance™ E. coli/coliform Selective Agar (formerly Chromogenic E. coli/coliform Selective Agar) is for the detection and enumeration of Escherichia coli and other coliforms from food and water samples.

Description

Brilliance E.coli/coliform agar is a differential agar used for the presumptive identification of Escherichia coli and coliforms from food and environmental samples. The agar base uses two enzyme substrates to differentiate between Escherichia coli and other coliforms. One chromogenic substrate is cleaved by the enzyme glucuronidase, which is specific for Escherichia coli and produced by approximately 97% of strains^1,2,3. The second chromogenic substrate is cleaved by galactosidase, an enzyme produced by the majority of coliforms. This results in purple Escherichia coli colonies, as they are able to cleave both chromogenic substrates, and pink coliform colonies, as they are only able to cleave the galactosidase chromogen. 

Technique

Dry the surface of the medium. Prepare the food sample by diluting as appropriate with 0.1% sterile peptone water (CM0009) and homogenise in a stomacher or laboratory blender. Pipette 0.5ml or 1.0ml of the homogenate, as appropriate, on to the plate, and spread over the surface with a glass spreader. Incubate the plates for 18-24 hours at 37°C.

Calculate the total number of coliforms per gram by multiplying purple and pink colonies by the dilution factor. The number of presumptive Escherichia coli is obtained by multiplying the number of purple colonies by the dilution factor.

Storage conditions and Shelf life

Store the dehydrated medium at 10-30°C, and use before the expiry date on the label.

Store the prepared medium at 2-8°C.

Appearance

Dehydrated medium; Straw to straw/pink free flowing powder

Prepared medium; Opaque pink coloured gel.

รายละเอียดสินค้า

รหัสสินค้า : CM1046

ยี่ห้อ : Oxoid

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