MODIFIED SEMI-SOLID RAPPAPORT VASSILIADIS AGAR (ISO)

Modified Semi-solid Rappaport-Vassiliadis (MSRV) Agar (ISO) is used for the selective enrichment of Salmonella spp. from food, animal faeces and environmental samples from the primary stage of animal production. This medium meets the specifications for formulation and performance described in ISO 6579:2002 +A1:2007Annex D¹.

Directions

Suspend 31.6g of MSRV (ISO) Agar base in 1 litre of distilled water. Bring to the boil with frequent agitation. DO NOT AUTOCLAVE. Cool to 50°C, and aseptically add the contents of 1 vial of Novobiocin Supplement (SR0181), reconstituted as directed. Mix well, and pour into sterile Petri dishes. This medium is semi-solid, therefore do not invert plates.

Description

Modified Semi-solid Rappaport Vassiliadis Agar (ISO) is based on the formulation described by De Smedt et al., which has been shown to detect more Salmonella-positive samples than traditional enrichment procedures^2,3. Further collaborative studies have confirmed these findings^4,5.

Motility enrichment on MSRV Agar has been designed as a simple and sensitive method for the isolation of salmonellae from food and environmental samples. It has been incorporated into ISO 6579:2002 +A1:2007 Annex D¹, which applies to the isolation of Salmonella spp. from animal faeces (such as poultry, pigs and cattle) and environmental samples associated with the primary stage of animal production (such as dust). In Annex D, MSRV Agar replaces Rappaport-Vassiliadis Broth, which is still used in ISO 6579:2002 +A1:2007 for the selective enrichment of Salmonella spp. in food.

The efficiency of the medium is based on the ability of salmonellae to migrate through the selective medium ahead of competing Gram-negative, motile organisms, thus producing opaque halos of growth. Novobiocin, low pH, high magnesium chloride concentration and malachite green inhibit the growth of Gram-positive flora.

Subculture should be carried out from the migrated culture, with the inoculum being taken from the furthest edge of the growth. Presumptive identification is achieved by subculture onto XLD Agar and a second Salmonella agar of choice. Characteristic presumptive Salmonella colonies should be confirmed according to directions in ISO 6579:2002 +A1:2007 Annex D¹. Alternatively, Oxoid Salmonella Latex Test FT0203 or DR1108 may be used for serological confirmation of Salmonella spp. MSRV (ISO) Agar is not suitable for the detection of non-motile strains of Salmonella (clinical incidence <0.1%)⁶.

Storage conditions and Shelf life

MSRV (ISO) should be stored in the tightly capped original container at 10-30°C. Novobiocin Supplement should be stored in the dark at 2-8°C. When stored as directed, the un-opened products will remain stable until the expiry date printed on the packaging.

Locally prepared MSRV (ISO) plates can be stored for up to 2 weeks when made from CM1112 and SR0181 according to the manufacturer’s instructions and stored at 2-8°C, out of direct sunlight. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage instructions.

Appearance

Dehydrated culture medium: Blue/green, free-flowing powder

Selective Supplement: White, freeze-dried pellet

Prepared medium: Blue, semi-solid gel

รายละเอียดสินค้า

รหัสสินค้า : CM1112

ยี่ห้อ : Oxoid

Documents & Downloads

Technical Data Sheet คลิกที่นี่

MSDS คลิกที่นี่

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