RAPPAPORT-VASSILIADIS SOYA PEPTONE BROTH (RVS BROTH)

A selective enrichment broth for the isolation of salmonellae.

Directions

Suspend 26.75g in 1 litre of distilled water and heat gently to dissolve. Dispense 10ml volumes into screw-capped bottles or tubes and sterilise by autoclaving at 115°C for 15 minutes.

Description

Rappaport-Vassiliadis Soya Peptone Broth (RSV Broth) is recommended as a selective enrichment medium for the isolation of salmonellae from food and environmental specimens. It shares with the original formulation¹, the ability to exploit the full characteristics of Salmonella species when compared with other Enterobacteriaceae. These are:

1. The ability to survive at relatively high osmotic pressure.

2. To multiply at relatively low pH values.

3. To be relatively more resistant to malachite green.

4. To have relatively less demanding nutritional requirements.

RVS Broth is based on the revised formulation described by van Schothorst et al.², and is recommended as the selective enrichment medium for the isolation of salmonellae from food and environmental specimens. It can also be used to isolate salmonellae from human faeces without the need for pre-enrichment. It is a modification of the Rappaport Vassiliadis (RV) Enrichment Broth described earlier by van Schothorst and Renauld³. The modifications to their earlier formula are:

1. The addition of di-potassium hydrogen phosphate to buffer the medium so that the pH is maintained during storage of the prepared broth.

2. Clarifying the optimum concentration of magnesium chloride 6H₂O.

The two modifications are said to enhance the reliability of the enrichment broth¹. Peterz et al.⁴ have also highlighted the importance of the concentration of magnesium chloride in the final medium.

Storage conditions and Shelf life

Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Store the prepared medium at 2-8°C.

Appearance

Dehydrated medium: Straw/green coloured, free-flowing coarse powder

Prepared medium: Blue coloured solution

Quality control

รายละเอียดสินค้า

รหัสสินค้า : CM0866

ยี่ห้อ : Oxoid

Documents & Downloads

Technical Data Sheet คลิกที่นี่

MSDS คลิกที่นี่

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