THIOGLYCOLLATE MEDIUM (BREWER)

An anaerobic medium especially useful for the sterility control of solutions containing mercury preservatives.

Directions

Suspend 20g in 1 litre of distilled water. Bring to the boil, mix well and allow to stand until completely dissolved. Distribute into final containers and sterilise by autoclaving at 121°C for 15 minutes.

Media containing small quantities of agar are liable to separate if cooled rapidly. Tubes of reconstituted and autoclaved medium should be allowed to cool slowly on a wooden surface in a draught-free atmosphere.

Description

This anaerobic medium, developed by Brewer^1,2,3 is used principally for testing the sterility of biological products.

The medium contains a small concentration of methylene blue as an oxidation-reduction indicator. If more than 20% of the uppermost portion of the stored medium has changed to a green colour, anaerobic conditions may be restored by heating in a boiling water bath or steamer for 5 to 10 minutes. This treatment must not be repeated.

Thioglycollate Medium (Brewer) is especially useful for the control of biological solutions containing mercurial preservatives, the toxicity of the latter being neutralised by the thioglycollate.

Storage conditions and Shelf life

Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

The prepared medium should be stored away from light at room temperature.

Appearance

Dehydrated medium: Straw coloured, free-flowing powder

Prepared medium: Straw/yellow coloured solution with green layer

Quality control

รายละเอียดสินค้า

รหัสสินค้า : CM0023

ยี่ห้อ : Oxoid

Documents & Downloads

Technical Data Sheet คลิกที่นี่

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