UREA AGAR BASE

An agar base for the preparation of Christensen’s medium to detect rapid urease activity of the Proteae and non-rapid urease activity of some Enterobacteriaceae.

Directions

Suspend 2.4g in 95ml of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 115°C for 20 minutes. Cool to 50°C and aseptically introduce 5ml of sterile 40% Urea Solution SR0020. Mix well, distribute 10ml amounts into sterile containers and allow to set in the slope position.

Description

Urea Agar Base is recommended for the preparation of Christensen medium¹ for the detection of rapid urease activity of the urease-positive Proteae. The urea medium may be used for the detection of urea hydrolysis by some other Enterobacteriaceae but the incubation period is much longer 24-48 hours.

Technique

Heavily inoculate the surface of a Urea Agar slope with a pure culture of the organism to be tested. When inoculated with urease-positive Proteae the reaction is usually complete after 3-5 hours at 35°C: urease-producing organisms hydrolyse the urea to form ammonia, and the medium changes from orange to pink. 40% Urea Solution is supplied, as a sterile solution in ampoules, for the convenient preparation of this medium.

Storage conditions and Shelf Life

Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Store the prepared medium at 2-8°C.

Appearance

Dehydrated medium: Straw coloured, free-flowing powder

Prepared medium: Orange coloured gel

Quality control

รายละเอียดสินค้า

รหัสสินค้า : CM0053

ยี่ห้อ : Oxoid

Documents & Downloads

Technical Data Sheet คลิกที่นี่

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