X.L.D. AGAR

A selective medium for the isolation of salmonellae and shigellae from clinical specimens and foods.

Directions

Suspend 53g in 1 litre of distilled water. Heat with frequent agitation until the medium boils. DO NOT OVERHEAT. Transfer immediately to a water bath at 50°C. Pour into sterile Petri dishes as soon as the medium has cooled.

It is important to avoid preparing large volumes which will cause prolonged heating.

Description

Xylose-Lysine-Desoxycholate Agar was originally formulated by Taylor1 for the isolation and identification of shigellae from stool specimens. It has since been found to be a satisfactory medium for the isolation and presumptive identification of both salmonellae and shigellae2. It relies on xylose fermentation, lysine decarboxylation and production of hydrogen sulphide for the primary differentiation of shigellae and salmonellae from non-pathogenic bacteria.

Rapid xylose fermentation is almost universal amongst enteric bacteria, except for members of the Shigella, Providencia and Edwardsiella genera1. Xylose is thus included in the medium so that Shigella spp. may be identified by a negative reaction.

Storage conditions and Shelf life

Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Store the prepared medium at 2-8°C.

Appearance

Dehydrated medium: Straw-pink coloured, free-flowing powder

Prepared medium: Red coloured gel

รายละเอียดสินค้า

รหัสสินค้า : CM0469

ยี่ห้อ : Oxoid

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