YEAST AND MOULD AGAR

A medium for the isolation of yeasts and moulds.

Directions

Suspend 41.0g in 1 litre of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. The medium may be rendered selective after sterilisation by acidifying to pH 4.0 with 12-15ml of Lactic Acid SR0021 after cooling to 50°C. Do not reheat after making this addition. Mix well and pour into sterile Petri dishes.

Description

Yeast and Mould Agar is based on the formulation described by Wickerham^1,2. The medium is recommended for the isolation and maintenance of yeasts and moulds.

Yeast and Mould Agar may be rendered selective by the addition of acid to reduce the pH of the medium to pH 4.0. A suitable acid for use is Lactic Acid SR0021.

Yeast and Mould Agar has now been formulated specifically for suitability within the Brewing Industry, for detection of both saccharomyces and non saccharomyces wild yeasts in the presence of culture yeast.

The use of MYGP + Copper Medium was described by Taylor and Marsh for selective isolation of wild yeasts in the presence of culture ale or larger yeasts³.

The medium utilises the the different sensitivities of wild yeast vs.culture ale or lager yeast to the inhibitory action of copper. This enables wild yeasts to grow on the medium whilst suppressing growth of culture yeast.

Technique

Cultures on plates or membrane filters are incubated for 3 days at 25°C under strictly anaerobic conditions. Continue incubation of the cultures under aerobic conditions for a further 2 days. Yeast colonies may be very small immediately following anaerobic incubation but will increase in size in air. Mould growth may become completely unrestricted after 3 days in air.

Storage conditions and Shelf life

Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

The prepared medium may be stored for up to 2 weeks at 2-8°C.

Appearance

Dehydrated medium: Straw coloured, free-flowing powder

Prepared medium: Straw coloured gel

Quality control

รายละเอียดสินค้า

รหัสสินค้า : CM0920

ยี่ห้อ : Oxoid

Documents & Downloads

Technical Data Sheet คลิกที่นี่

MSDS คลิกที่นี่

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