MSRV SELECTIVE SUPPLEMENT

Directions

Suspend 15.8g of MSRV Medium Base in 500ml of distilled water. Bring to the boil with frequent agitation.

DO NOT AUTOCLAVE. Cool to 50°C and aseptically add the contents of 1 vial of MSRV Selective Supplement SR0161E reconstituted with 2ml of sterile distilled water. Mix well and pour into sterile Petri dishes. Air dry at room temperature for at least one hour. (Plates may be air-dried overnight prior to storage at 2-8°C.)

Description

Modified Semi-solid Rappaport Vassiliadis (MSRV) Medium is based on the formulation described by De Smedt et al. which has been shown to detect more Salmonella-positive samples than the traditional enrichment procedures^1,2. Further collaborative studies have confirmed these findings^3,4.

Motility enrichment on MSRV Medium has been designed as a simple, sensitive method for the isolation of salmonellae from food and environmental samples. The efficiency of the medium is based on the ability of salmonellae to migrate through the selective medium ahead of competing motile organisms, thus producing opaque halos of growth.

Further tests can be carried out directly from the migrated culture with the inoculum being taken from the edge of the growth. Oxoid Salmonella Latex Test FT0203 or DR1108 may be used for serological confirmation of Salmonella species.

The medium is not suitable for the detection of non-motile strains of Salmonella (incidence5. (Figures obtained from records of the Department of Enteric Pathogens, Central Public Health Laboratory, Colindale, London. Dr. B. Rowe, Personal Communication, 1988.)

Storage conditions and Shelf life

Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Store the selective supplement in the dark at 2-8°C and use before the expiry date on the label.

The prepared medium may be stored for up to 2 weeks at 2-8°C away from the light.

Appearance

Dehydrated medium: Green coloured, free-flowing powder

Prepared medium: Blue coloured semi-solid gel

Quality control

รายละเอียดสินค้า

รหัสสินค้า : SR0161

ยี่ห้อ : Oxoid

Documents & Downloads

Technical Data Sheet คลิกที่นี่

MSDS คลิกที่นี่

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