STREPTOCOCCUS SELECTIVE SUPPLEMENT (COBA)

Directions

Reconstitute one vial as directed, aseptically add the contents to 500ml of sterile Columbia Blood Agar Base containing 5% Defibrinated Horse Blood SR0050 cooled to approximately 50°C. Mix gently and pour into sterile Petri dishes.

Description

Streptococcus Selective Supplement is based on the formulation of Petts (COBA Medium)¹ and is recommended for the selective isolation of

streptococci of medical and veterinary importance.

COBA Medium possesses advantages over other media described for selective isolation of streptococci.

Agents previously recommended for inhibition of Gram-positive organisms can be shown to have severe effects on streptococci even at subminimal

inhibitory concentrations. The antibiotics gentamicin^2,3,4,5,6, amikacin⁷, fucidic acid⁸, neomycin⁹ and cotrimoxazole¹⁰ have all been shown to have

adverse effects as have the long established inhibitors crystal violet and sodium azide. Both colistin and oxolinic acid are thermostable and can, if necessary,

be stored without refrigeration.

Streptococci are commonly isolated from the upper respiratory tract. They are also often isolated from burns and other sites where frequently there is an

abundance of competing organisms. In order to isolate streptococci, especially when present in small numbers, it is necessary to inhibit the competing flora

without any adverse effect by the selective agents upon the Streptococcus species. The selective agents colistin sulphate (10 mg/ml) and oxolinic acid

(5 mg/ml) have been found to have no inhibitory effect on Streptococcus species although amongst Group D organisms Enterococcus faecalis colonies are somewhat smaller. The combination of these two selective agents results in total inhibition of Gram-negative organisms and almost all non-streptococcal Gram-positive organisms. A very few staphylococci and coryneform organisms may grow with reduced colony size. The haemolytic reactions on media containing blood are clearly defined, and the colonial size and growth recovery of streptococcal groups A, B, C, D and G and S. pneumoniae are comparable to that on a nonselective medium. The selective agents can also be used with Islam’s Medium (GBS Agar CM0755) for the isolation of Group B streptococci without loss of pigmentation occurring.

Storage conditions and Shelf life

Store the dehydrated medium at 10-30°C and use before the expiry date on the label.

Store the prepared medium at 2-8°C.

Appearance

Dehydrated Medium: Straw coloured, free-flowing powder.

Prepared medium: Opaque red-coloured gel.

Quality control



รายละเอียดสินค้า

รหัสสินค้า : SR0126

ยี่ห้อ : Oxoid

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